Premium
Increasing the autotrophic growth of Chlorella USTB ‐01 via the control of bacterial contamination by Bdellovibrio USTB ‐06
Author(s) -
Li Y.,
Qiu F.,
Yan H.,
Wan X.,
Wang M.,
Ren K.,
Xu Q.,
Lv L.,
Yin C.,
Liu X.,
Zhang H.,
Mahmoud K.
Publication year - 2018
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.13682
Subject(s) - bdellovibrio , chlorella , bacteria , autotroph , biology , escherichia coli , microbiology and biotechnology , contamination , food science , algae , biochemistry , botany , ecology , genetics , gene
Aims (i) To obtain and identify the predatory bacteria for the control of contaminated bacteria and to promote the autotrophic growth of Chlorella USTB ‐01. (ii) To identify and measure the different cell numbers in microalgal culture using flow cytometer. Methods and Results A predatory bacterial strain was isolated using Escherichia coli BL 21 as a sole prey host, which was identified as Bdellovibrio USTB ‐06 by the analysis of 16S rDNA sequence. A flow cytometer was successfully used to identify and measure the cell numbers of Chlorella USTB ‐01, the contaminated bacteria and Bdellovibrio USTB ‐06 simultaneously in the autotrophic culture of Chlorella USTB ‐01 according to the identification of the different cell sizes. With the addition of Bdellovibrio USTB ‐06 at initial 10 4 plaque‐forming units per ml, the contaminated bacteria severely decreased by about five counts (in log 10 CFU per ml) and the growth of Chlorella USTB ‐01 was greatly increased by 37·0% compared with those of control respectively. Conclusions Bdellovibrio USTB ‐06 could effectively promote the growth of Chlorella USTB ‐01 via the killing of the contaminated bacteria. Significance and Impact of the Study Our study reveals a good biotechnology method to increase the growth of Chlorella USTB ‐01 which is very important in the industry of microalgal culture.