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Optimization and characterization of biosurfactant from Streptomyces griseoplanus NRRL ‐ ISP 5009 ( MS 1)
Author(s) -
Elkhawaga M.A.
Publication year - 2018
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.13665
Subject(s) - yeast extract , chemistry , food science , glycerol , streptomyces , antimicrobial , yeast , chromatography , bacteria , biochemistry , fermentation , biology , organic chemistry , genetics
Aims This work aimed to study, isolate, characterize and stabilize the biosurfactant isolated from actinomycetes found in petroleum contaminated soil. Methods and Results Optimized production of the biosurfactant from Streptomyces griseoplanus NRRL ‐ ISP 5009, SM 1 was obtained on day 6 at 30°C, pH 7, 150 rev min −1 , in glycerol yeast extract broth medium supplemented with cellulose, yeast extract and 1% NaCl. The stability of the biosurfactant produced was studied at different temperatures, pH and different concentrations of NaCl. The produced biosurfactant was extracted and purified. Conclusion Streptomyces griseoplanus NRRL ‐ ISP 5009, SM 1 isolated from oil contaminated soil produced a biosurfactant exhibiting emulsification activity. The produced biosurfactant is a mixture of carbohydrate, lipid and protein. It has promising characteristics, including a higher stability at alkaline pH than at acidic pH , a salinity of 1–3% and stable in the temperature range from 0 and 100°C. Also, the potential antimicrobial activity of the purified biosurfactant was recorded. Significance and Impact of the Study The research was focused on the isolation of a novel source of biosurfactants that have great importance in the manufacture of food, detergent, pharmaceutical and cosmetics.