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Prospective role of indigenous Exiguobacterium profundum PT 2 in arsenic biotransformation and biosorption by planktonic cultures and biofilms
Author(s) -
Andreasen R.,
Li Y.,
Rehman Y.,
Ahmed M.,
Meyer R.L.,
Sabri A.N.
Publication year - 2018
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.13636
Subject(s) - biosorption , biofilm , microbiology and biotechnology , biology , effluent , bacteria , chemistry , food science , environmental chemistry , environmental engineering , adsorption , organic chemistry , environmental science , genetics , sorption
Aims The aim of this study was to analyse arsenic (As) transformation and biosorption by indigenous As‐resistant bacteria both in planktonic and biofilm modes of growth. Methods and Results As‐resistant bacteria were isolated from industrial waste water and strain PT 2, and identified as Exiguobacterium profundum through 16S rRNA gene sequencing was selected for further study. As transformation and biosorption by E. profundum PT 2 was determined by HPLC ‐ ICP ‐ MS analysis. Planktonic cultures reduced 3·73 mmol l −1 As 5+ into As 3+ from artificial waste water effluent after 48‐h incubation. In case of biosorption, planktonic cultures and biofilms exhibited 25·2 and 29·4 mg g −1 biomass biosorption, respectively. As biosorption kinetics followed Freundlich isotherm and pseudo second‐order model. Biofilm formation peaked after 3 days of incubation, and in the presence of As stress, biofilm formation was significantly affected in contrast to control ( P < 0·05). Homogeneous nature of mature biofilms with an increased demand of nutrients was revealed by minimum roughness and maximum surface to biovolume ratio measured through CLSM analysis. Conclusion Indigenous As‐resistant E. profundum PT 2 was found capable of As transformation and biosorption both in the form of planktonic cultures and biofilms. Significance and Impact of the Study Indigenous biofilm forming E. profundum PT 2 revealing As biosorption and biotransformation potential is presented an eco‐friendly and cost‐effective source for As remediation that can be implemented for waste water treatment.