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Antibacterial isoeugenol coating on stainless steel and polyethylene surfaces prevents biofilm growth
Author(s) -
Nielsen C.K.,
Subbiahdoss G.,
Zeng G.,
Salmi Z.,
Kjems J.,
Mygind T.,
Snabe T.,
Meyer R.L.
Publication year - 2018
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.13634
Subject(s) - isoeugenol , pseudomonas fluorescens , biofilm , coating , listeria monocytogenes , materials science , polyethylene , microbiology and biotechnology , chemistry , chemical engineering , bacteria , composite material , organic chemistry , biology , eugenol , engineering , genetics
Aims Pathogenic bacteria can spread between individuals or between food items via the surfaces they share. Limiting the survival of pathogens on surfaces, therefore, presents an opportunity to limit at least one route of how pathogens spread. In this study, we propose that a simple coating with the essential oil isoeugenol can be used to circumvent the problem of bacterial transfer via surfaces. Methods and Results Two commonly used materials, stainless steel and polyethylene, were coated by physical adsorption, and the coatings were characterized by Raman spectroscopy, atomic force microscopy and water contact angle measurements. We quantified and visualized the colonization of coated and uncoated surfaces by three bacteria: Staphylococcus aureus, Listeria monocytogenes and Pseudomonas fluorescens . No viable cells were detected on surfaces coated with isoeugenol. Conclusions The isoeugenol coating prepared with simple adsorption proved effective in preventing biofilm formation on stainless steel and polyethylene surfaces. The result was caused by the antibacterial effect of isoeugenol, as the coating did not diminish the adhesive properties of the surface. Significance and Impact of the Study Our study demonstrates that a simple isoeugenol coating can prevent biofilm formation of S. aureus, L. monocytogenes and P. fluorescens on two commonly used surfaces.