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Detection of latent infections caused by Colletotrichum sp. in olive fruit
Author(s) -
Romero J.,
AgustíBrisach C.,
Santa Bárbara A.E.,
Cherifi F.,
Oliveira R.,
Roca L.F.,
Moral J.,
Trapero A.
Publication year - 2018
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.13610
Subject(s) - colletotrichum , sodium hydroxide , cultivar , inoculation , paraquat , horticulture , biology , chemistry , food science , microbiology and biotechnology , organic chemistry , biochemistry
Aims To set up a practical method to detect latent infections of Colletotrichum sp., the causal agent of olive anthracnose, on olives before the onset of disease symptoms. Methods and Results Freezing, sodium hydroxide (Na OH ), ethanol and ethylene treatments were evaluated to detect latent infections on inoculated and naturally infected olive fruit by Colletotrichum sp. as non‐hazardous alternatives to paraquat. Treatments were conducted using fruit of cultivars Arbequina and Hojiblanca. The disease incidence and T 50 were calculated. Dipping in Na OH 0·05% solution and the paraquat method were the most effective treatments on both inoculated and naturally infected fruit, although the value of T 50 was lower for the Na OH method than for the paraquat method in one of the experiments. Subsequently, the dipping time in Na OH 0·05% was evaluated. Longer dipping times in Na OH 0·05% were better than shorter ones in cultivar Arbequina, with 72 h being the most effective in cultivar Hojiblanca. Conclusions Na OH solution is a practical method to detect latent infections of Colletotrichum sp. on immature olive fruit. Significance and Impact of the Study This study is relevant because we set up a viable, non‐hazardous alternative to paraquat to detect latent infections of Colletotrichum sp. using Na OH . The use of Na OH is a simple and eco‐friendly tool that allows the determination of the level of latent infections by Colletotrichum in olives. Therefore, our method will be useful in decision‐making processes for disease management before the appearance of the first visible symptoms.