Premium
Evaluation of air samplers and filter materials for collection and recovery of airborne norovirus
Author(s) -
Uhrbrand K.,
Koponen I. K.,
Schultz A. C.,
Madsen A. M.
Publication year - 2018
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.13588
Subject(s) - aerosolization , norovirus , environmental science , filter (signal processing) , sampling (signal processing) , microplastics , chromatography , aerosol , pulp and paper industry , murine norovirus , bioaerosol , waste management , chemistry , environmental chemistry , biology , virology , engineering , virus , organic chemistry , anatomy , inhalation , electrical engineering
Abstract Aims The aim of this study was to identify the most efficient sampling method for quantitative PCR ‐based detection of airborne human norovirus (NoV). Methods and Results A comparative experiment was conducted in an aerosol chamber using aerosolized murine norovirus ( MNV ) as a surrogate for NoV. Sampling was performed using a nylon ( NY ) filter in conjunction with four kinds of personal samplers: Gesamtstaubprobenahme sampler ( GSP ), Triplex‐cyclone sampler ( TC ), 3‐piece closed‐faced Millipore cassette (3P) and a 2‐stage NIOSH cyclone sampler ( NIO ). In addition, sampling was performed using the GSP sampler with four different filter types: NY , polycarbonate ( PC ), polytetrafluoroethylene ( PTFE ) and gelatine ( GEL ). The sampling efficiency of MNV was significantly influenced by both sampler and filter type. The GSP sampler was found to give significantly ( P < 0·05) higher recovery of aerosolized MNV than 3P and NIO . A higher recovery was also found for GSP compared with TC , albeit not significantly. Finally, recovery of aerosolized MNV was significantly ( P < 0·05) higher using NY than PC , PTFE and GEL filters. Conclusions The GSP sampler combined with a nylon filter was found to be the best method for personal filter‐based sampling of airborne NoV. Significance and Impact of the Study The identification of a suitable NoV air sampler is an important step towards studying the association between exposure to airborne NoV and infection.