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Synthesizing value‐added products from methane by a new Methylomonas
Author(s) -
Guo W.,
Li D.,
He R.,
Wu M.,
Chen W.,
Gao F.,
Zhang Z.,
Yao Y.,
Yu L.,
Chen S.
Publication year - 2017
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.13581
Subject(s) - carotenoid , methanol , chemistry , abts , food science , agaricus bisporus , polysaccharide , chromatography , mushroom , biochemistry , organic chemistry , dpph , antioxidant
Aims Methane and methanol are potential carbon sources of industrial micro‐organisms in addition to crop‐derived bio‐carbon sources. Methanotrophs that can utilize these simple, stable and large amounts chemicals are expected to be developed into ‘cell factories’ for the production of specific chemicals. In this study, a methanotroph that can synthesize lycopene, C 30 carotenoid and exopolysaccharides (EPS) with relative better performances from C1 substrates was isolated, and its performances were evaluated. Methods and Results The isolated strain was identified as Methylomonas sp. ZR 1 based on 16S rRNA sequence analysis. Its maximum specific growth rate achieved 0·200 h −1 under flask culture conditions, and 0·386 h −1 in bubble column reactors. ZR 1 was able to utilize 35 g l −1 of methanol and even exhibited slight growth in the presence of 40 g l −1 of methanol. Furthermore, ZR 1 was proved to synthesize lycopene (C 40 carotenoids) besides the C 30 carotenoids through HPLC ‐ DAD and HPLC ‐ MS / MS analysis methods. Its carotenoid extracts exhibited excellent antioxidative activities measured by the ABTS + method. Plenty of polysaccharides were also synthesized by ZR 1, the components of the polysaccharides were identified as glucose, mannose and galactose with a proportion of 1 : 2 : 1 by GC ‐ MS , and its yield achieved 0·13 g g −1 cell dry weight. Conclusions The isolated strain has great potential for the production of value‐added bioproducts from C1 compounds because of its excellent C1 substrate utilizing abilities and its abilities to naturally synthesize lycopene, C 30 carotenoids and EPS. Significance and Impact of the Study In this study, we isolated a fast‐growing methanotroph, its C1 carbon substrate utilizing ability is excellent in comparison with reported methanotrophs. Furthermore, besides polysaccharides and C 30 carotenoids which were commonly synthesized by methanotrophs, our findings suggested that C 40 lycopene could also be naturally synthesized from methane by methanotrophs.