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Functional analysis of the biosynthetic gene cluster required for immunity and secretion of a novel Lactococcus ‐specific bacteriocin, lactococcin Z
Author(s) -
Daba G.M.,
Ishibashi N.,
Zendo T.,
Sonomoto K.
Publication year - 2017
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.13564
Subject(s) - bacteriocin , biology , gene cluster , immunity , secretion , lactococcus lactis , lactococcus , heterologous , heterologous expression , gene , genetics , immune system , biochemistry , bacteria , recombinant dna , lactic acid
Aims Characterization of the biosynthesis (secretion and immunity) of lactococcin Z. Methods and Results Lactococcin Z is produced by Lactococcus lactis QU 7. DNA sequence analysis revealed that the lactococcin Z gene cluster ( c . 5·1 kb) includes four genes encoding putative biosynthetic proteins, LczB (self‐immunity protein), LczC (an ABC transporter) and LczD (a transport accessory protein), besides the previously identified LczA. LczB showed 25·5% identity to LciA, the lactococcin A immunity protein, while LczC and LczD had 93·7 and 95·3% identities, respectively, to corresponding proteins of lactococcin A. Heterologous expression of various combinations of the four genes indicated that lczB confers self‐immunity against lactococcin Z, and that the four genes are necessary to produce lactococcin Z. However, LczB and LciA showed no cross‐immunity to lactococcins A and Z respectively. Conclusions The results verified that LczB is the lactococcin Z immunity protein, and LczC is responsible for lactococcin Z secretion in a manner dependent on LczD expression. Significance and Impact of the Study The biosynthesis (secretion and immunity) of a new Lactococcus ‐specific bacteriocin, lactococcin Z, was characterized. Moreover, the results suggested that lactococcin Z has different immunity and action mechanisms from other Lactococcus ‐specific bacteriocins.