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Multi‐effect of the water‐soluble Moringa oleifera lectin against Serratia marcescens and Bacillus sp.: antibacterial, antibiofilm and anti‐adhesive properties
Author(s) -
Moura M.C.,
Trentin D.S.,
Napoleão T.H.,
PrimonBarros M.,
Xavier A.S.,
Carneiro N.P.,
Paiva P.M.G.,
Macedo A.J.,
Coelho L.C.B.B.
Publication year - 2017
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.13556
Subject(s) - serratia marcescens , moringa , microbiology and biotechnology , serratia , lectin , antibacterial activity , chemistry , bacteria , biology , pseudomonas , food science , escherichia coli , biochemistry , genetics , gene
Aims To evaluate the antibiofilm potential of water‐soluble Moringa oleifera seed lectin ( WSM oL) on Serratia marcescens and Bacillus sp. Methods and Results WSM oL inhibited biofilm formation by S. marcescens at concentrations lower than 2·6 μ g ml −1 and impaired bacterial growth at higher concentrations, avoiding biofilm formation. For Bacillus sp., the lectin inhibited bacterial growth at all concentrations. The antibiofilm action of WSM oL is associated with damage to bacterial cells. WSM oL did not disrupt preformed S. marcescens biofilms but was able to damage cells inside them. On the other hand, the lectin reduced the number of cells in Bacillus sp. biofilm treated with it. WSM oL was able to control biofilm formation when immobilized on glass surface (116 μ g cm −2 ), damaging S. marcescens cells and avoiding adherence of Bacillus sp. cells on glass. The Bacillus sp. isolate is member of Bacillus subtilis species complex and closely related to species of the conspecific ‘ amyloliquefaciens ’ group. Conclusion WSM oL prevented biofilm development by S. marcescens and Bacillus sp. and the antibiofilm effect is also observed when the lectin is immobilized on glass. Significance and Impact of the Study Taking together, our results provide support to the potential use of WSM oL for controlling biofilm formation by bacteria.