Premium
The antibacterial activity and modes of LI ‐F type antimicrobial peptides against Bacillus cereus in vitro
Author(s) -
Han J.,
Zhao S.,
Ma Z.,
Gao L.,
Liu H.,
Muhammad U.,
Lu Z.,
Lv F.,
Bie X.
Publication year - 2017
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.13526
Subject(s) - bacillus cereus , antimicrobial , cereus , bacteria , bacterial cell structure , antimicrobial peptides , nucleic acid , microbiology and biotechnology , biology , membrane permeability , cell wall , biochemistry , chemistry , membrane , genetics
Aims LI ‐Fs are a family of highly potent cyclic lipodepsipeptide antibiotics with a broad antimicrobial spectrum (Gram‐positive bacteria and fungi). In this study, LI ‐F‐type antimicrobial peptides ( AMP ‐jsa9) composing of LI ‐F03a, LI ‐F03b, LI ‐F04a, LI ‐F04b and LI ‐F05b were isolated from Paenibacillus polymyxa JSA ‐9. To better understand the antimicrobial mechanism of AMP ‐jsa9, the potency and action(s) of AMP ‐jsa9 against Bacillus cereus were examined. Methods and Results Flow cytometry, confocal laser microscopy, scanning electron microscopy, transmission electron microscopy ( TEM ) and atomic force microscopy observation, as well as determination of peptidoglycan and cell wall‐associated protein and other methods were used. The results indicate that AMP ‐jsa9 exhibits strong, broad‐spectrum antimicrobial activity. Moreover, AMP ‐jsa9 targets the cell wall and membrane of B. cereus to impair membrane integrity, increase membrane permeability and enhance cytoplasm leakage (e.g. K + , protein, nucleic acid). This leads to bacterial cells with irregular, withered and coarse surfaces. In addition, AMP ‐jsa9 is also able to bind to DNA and break down B. cereus biofilms. Conclusions In this study, the action mechanism of LI ‐Fs against B. cer eus was clarified in details. Significance and Impact of the Study The results of this study provide a theoretical basis for utilizing AMP ‐jsa9 or similar analogues as natural and effective preservatives in the food and feed industries. These efforts could also stimulate research activities interested in understanding the specific effects of other antimicrobial agents.