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Development of immunochromatographic colloidal gold test strip for rapid detection of Haemophilus influenzae in clinical specimens
Author(s) -
Kong M.M.,
Yang B.,
Gong C.J.,
Wang H.,
Li X.,
Zhao K.S.,
Li J.J.,
Wu F.,
Liu X.,
Hu Z.
Publication year - 2017
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.13489
Subject(s) - haemophilus influenzae , mycoplasma pneumoniae , colloidal gold , streptococcus pneumoniae , microbiology and biotechnology , moraxella catarrhalis , gold standard (test) , detection limit , legionella , materials science , chemistry , virology , chromatography , medicine , bacteria , nanotechnology , biology , pneumonia , nanoparticle , genetics , antibiotics
Aims A simple and fast test strip for simultaneous detection of Haemophilus influenzae, based on the theory of colloidal gold immunochromatography was developed. Methods and Results In this study, monodispersional colloidal gold and gold‐labelled polyclonal antibody (AbP6Line) specific to the linear antigenic determinant OMP 6 of H. influenzae were prepared, and identified by UV ‐visible spectroscopy (200–700 nm) and transmission electron microscopy. The test strip was assembled on a plastic backing containing a sample pad, a conjugate pad, an absorbent pad and a nitrocellulose membrane, on which test and control lines were stuck. The strip showed us specific recognization of H. influenzae and did not identify other common respiratory pathogens such as Streptococcus pneumoniae , Mycoplasma pneumoniae , Moraxella catarrhalis and Legionella pneumophila . It was shown that the detection limit of the test strip was as low as 1 × 10 6 CFU per ml and the whole process can be completed within 10 min. The strips could be stored at 4°C for at least 6 months without losing sensitivity or specificity. Conclusions The test strip was specific, simple and convenient for rapid detection of H. influenzae presenting good stability and reproducibility. Significance and Impact of the Study The test strip provides a simple, convenient and specific tool for the on‐site surveillance and diagnosis of H. influenzae infection in clinical samples.