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Cytosolic expression of functional Fab fragments in Escherichia coli using a novel combination of dual SUMO expression cassette and EnBase ® cultivation mode
Author(s) -
Rezaie F.,
Davami F.,
Mansouri K.,
Agha Amiri S.,
Fazel R.,
Mahdian R.,
Davoudi N.,
Enayati S.,
Azizi M.,
Khalaj V.
Publication year - 2017
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.13483
Subject(s) - escherichia coli , fusion protein , mutant , recombinant dna , biology , expression vector , chemistry , biochemistry , microbiology and biotechnology , gene
Aims The Escherichia coli expression system is highly effective in producing recombinant proteins. However, there are some limitations in this system, especially in obtaining correctly folded forms of some complex proteins such as Fab fragments. To improve the solubility and folding quality of Fab fragments, we have examined the effect of simultaneous application of a SUMO fusion tag, EnBase ® cultivation mode and a redox mutant strain in the E. coli expression system. Methods and Results A bicistronic gene construct was designed to express an antivascular endothelial growth factor (VEGF) Fab fragment as a model system. The construct contained a dual SUMO fusion gene fragment to encode SUMO‐tagged heavy and light chains. While the expression of the construct in batch cultures of BL21 or SHuffle ® transformants produced insoluble and unfolded products, the induction of the transformants in EnBase ® medium resulted in soluble and correctly folded Fab fragment, reaching as high as 19% of the total protein in shuffle strain. The functional assays indicated that the biological activity of the target Fab is similar to the commercial anti‐VEGF, Lucentis ® . Conclusions This study demonstrated that the combination of SUMO fusion technology, EnBase ® cultivation system and recruiting a redox mutant of E. coli can efficiently enhance the solubility and productivity of recombinant Fab fragments. Significance and the Impact of the study The presented strategy provides not only a novel method to produce soluble and active form of an anti‐VEGF Fab but also may use in the efficient production of other antibody fragments.