Lactobacillus paracasei  metabolism of rice bran reveals metabolome associated with  Salmonella  Typhimurium growth reduction | Zendy

Nealon N.J. | Zendy; Worcester C.R. | Zendy; Ryan E.P. | Zendy

Premium

Lactobacillus paracasei metabolism of rice bran reveals metabolome associated with Salmonella Typhimurium growth reduction

Author(s) -

Nealon N.J.,

Worcester C.R.,

Ryan E.P.

Publication year - 2017

Publication title -

journal of applied microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.889

H-Index - 156

eISSN - 1365-2672

pISSN - 1364-5072

DOI - 10.1111/jam.13459

Subject(s) - metabolite , metabolome , lactobacillus paracasei , salmonella , antimicrobial , probiotic , microbiology and biotechnology , metabolomics , food science , biology , chemistry , bacteria , biochemistry , chromatography , genetics

Aims This study aimed to determine the effect of a cell‐free supernatant of Lactobacillus paracasei ATCC 27092 with and without rice bran extract ( RBE ) on Salmonella Typhimurium 14028s growth, and to identify a metabolite profile with antimicrobial functions. Methods and Results Supernatant was collected from overnight cultures of L. paracasei incubated in the presence ( LP + RBE ) or absence ( LP ) of RBE and applied to S . Typhimurium. LP + RBE reduced 13·1% more S . Typhimurium growth than LP after 16 h ( P < 0·05). Metabolite profiles of LP and LP + RBE were examined using nontargeted global metabolomics consisting of ultra‐high‐performance liquid chromatography coupled with tandem mass spectrometry. A comparison of LP and LP + RBE revealed 84 statistically significant metabolites ( P < 0·05), where 20 were classified with antimicrobial functions. Conclusions LP + RBE reduced S . Typhimurium growth to a greater extent than LP , and the metabolite profile distinctions suggested that RBE favourably modulates the metabolism of L. paracasei . These findings warrant continued investigation of probiotic and RBE antimicrobial activities across microenvironments and matrices where S . Typhimurium exposure is problematic. Significance and Impact of the Study This study showed a novel metabolite profile of probiotic L. paracasei and prebiotic rice bran that increased antimicrobial activity against S . Typhimurium.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research