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Lactobacillus paracasei metabolism of rice bran reveals metabolome associated with Salmonella Typhimurium growth reduction
Author(s) -
Nealon N.J.,
Worcester C.R.,
Ryan E.P.
Publication year - 2017
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.13459
Subject(s) - metabolite , metabolome , lactobacillus paracasei , salmonella , antimicrobial , probiotic , metabolomics , microbiology and biotechnology , food science , biology , chemistry , bacteria , biochemistry , lactobacillus , fermentation , chromatography , genetics
Aims This study aimed to determine the effect of a cell‐free supernatant of Lactobacillus paracasei ATCC 27092 with and without rice bran extract ( RBE ) on Salmonella Typhimurium 14028s growth, and to identify a metabolite profile with antimicrobial functions. Methods and Results Supernatant was collected from overnight cultures of L. paracasei incubated in the presence ( LP + RBE ) or absence ( LP ) of RBE and applied to S . Typhimurium. LP + RBE reduced 13·1% more S . Typhimurium growth than LP after 16 h ( P < 0·05). Metabolite profiles of LP and LP + RBE were examined using nontargeted global metabolomics consisting of ultra‐high‐performance liquid chromatography coupled with tandem mass spectrometry. A comparison of LP and LP + RBE revealed 84 statistically significant metabolites ( P < 0·05), where 20 were classified with antimicrobial functions. Conclusions LP + RBE reduced S . Typhimurium growth to a greater extent than LP , and the metabolite profile distinctions suggested that RBE favourably modulates the metabolism of L. paracasei . These findings warrant continued investigation of probiotic and RBE antimicrobial activities across microenvironments and matrices where S . Typhimurium exposure is problematic. Significance and Impact of the Study This study showed a novel metabolite profile of probiotic L. paracasei and prebiotic rice bran that increased antimicrobial activity against S . Typhimurium.