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The trans ‐10, cis ‐12 conjugated linoleic acid increases triacylglycerol hydrolysis in yeast Saccharomyces cerevisiae
Author(s) -
Hokkanen S.,
Laakso S.,
Senn C.M.,
Frey A.D.
Publication year - 2017
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.13443
Subject(s) - conjugated linoleic acid , yeast , saccharomyces cerevisiae , biochemistry , lipid metabolism , enzyme , metabolism , linoleic acid , hydrolysis , chemistry , biology , fatty acid
Aims The trans ‐10, cis ‐12 conjugated linoleic acid ( CLA ) is known for its antilipogenic effect but the mechanism is not fully clear. In this study, the potential of yeast ( Saccharomyces cerevisiae ) metabolism to offer evidence for the mechanism was investigated. Methods and Results The inhibitory effect of CLA on lipid accumulation was studied by analysing the transcript abundance of selected genes involved in triacylglycerol synthesis ( LRO 1 , DGA 1 , ARE 1 and ARE 2 ) in the presence of the two bioactive CLA isomers: trans‐ 10, cis‐ 12 and the cis ‐9, trans ‐11 CLA . None of the enzymes was reduced in transcription but the expression of ARE 2 was induced by trans‐ 10, cis‐ 12 CLA . However, the ARE 2 overexpression did not contribute to lipid accumulation. The expression of the Δ9 desaturase gene, OLE 1 , was reduced by the cis‐ 9, trans‐ 11 but not by the trans‐ 10, cis‐ 12 isomer. In the TGL 3/ TGL 4 ‐knockout strain the triacylglycerol content also remained high in the CLA fed cells. Conclusions Triacylglycerol hydrolysis rather than synthesis was the most probable reason for the reduced lipid content in yeast induced by CLA . Significance and Impact of the Study This study revealed new aspects of the functionality of CLA in eukaryotic lipid metabolism. Yeast was proven to be an applicable model to study further the mechanism of trans‐ 10, cis‐ 12 CLA functionality on lipid metabolism.