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Application of bacteria from non‐cultivated plants to promote growth, alter root architecture and alleviate salt stress of cotton
Author(s) -
Irizarry I.,
White J.F.
Publication year - 2017
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.13414
Subject(s) - bacillus amyloliquefaciens , biology , malvaceae , germination , bacteria , seedling , inoculation , gossypium barbadense , crop , horticulture , agronomy , fiber crop , botany , gossypium hirsutum , genetics
Abstract Aims Cotton seeds are frequently treated with acid to remove fibres and reduce seed‐transmitted diseases. This process also eliminates beneficial bacteria on the seed surface. The goal of this research was to seek and apply beneficial bacteria to acid delinted cotton seeds to evaluate their growth‐promoting and salt stress alleviating effects in seedlings. Methods and Results Bacteria were isolated from non‐cultivated plants in the Malvaceae. Seeds were collected from Portia tree ( Thespesia populnea ) and wild cotton ( Gossypium hirsutum ) from coastal and arid areas of Puerto Rico. Bacillus amyloliquefaciens , Curtobacterium oceanosedimentum and Pseudomonas oryzihabitans were inoculated onto acid delinted cotton seeds. Bacteria increased cotton seed germination and length of emerging seedling radicles. Cotton seeds were inoculated with B. amyloliquefaciens to evaluate growth and root architecture of non‐stressed and salt stressed seedlings. Inoculating cotton seeds with B. amyloliquefaciens led to a greater percentage of seedlings with expanded cotyledons after 8 days, enhanced primary and lateral root growth, and altered root architecture. Similar results were obtained when okra seeds were inoculated with B. amyloliquefaciens . Conclusion The data supported the hypothesis that non‐cultivated plants in the Malvaceae growing in stressful environments possess bacteria that promote growth, alter root architecture and alleviate salt stress of cotton and okra seedlings. Significance and Impact of the Study This study demonstrated the effects of applying beneficial bacteria on acid delinted cotton seeds. Inoculating seeds with salt stress alleviating bacteria could improve the growth of crop seedlings that are vulnerable to soil salinization.