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Low prevalence of Aichi virus in molluscan shellfish samples from Galicia ( NW Spain)
Author(s) -
Rivadulla E.,
Varela M.F.,
Romalde J.L.
Publication year - 2017
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.13363
Subject(s) - sapovirus , shellfish , norovirus , biology , veterinary medicine , virus , virology , fishery , aquatic animal , fish <actinopterygii> , medicine
Abstract Aims The aim of this study was to detect and quantify Aichi virus (AiV) in shellfish from three estuaries in Galicia, the main producer of molluscs in Europe. Methods and Results A total of 249 shellfish samples were analysed using a reverse transcription‐quantitative PCR procedure. AiV was detected in 15 of 249 (6·02%) samples. Ría de Ares‐Betanzos showed the highest prevalence (11·1%), followed by Ría do Burgo (3·7%) and Ría de Vigo, (2·56%). AiV quantifications ranged from nonquantifiable (under the limit of quantification of the method) to 6·9 × 10 3 RNA c per g DT , with a mean value of 1·9 × 10 2 RNA c per g DT . Conclusion Results obtained indicated that the prevalence of this enteric virus in the studied area is considerably lower than those of other enteric viruses, such as Norovirus, Sapovirus, HAV or HEV . Significance and Impact of the Study This is the first study that detects the presence of AiV in shellfish from authorized harvesting areas in Spain. Further studies with clinical samples are needed to determine the potential risk of AiV for human health in Galicia.