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Immunization with r‐ Lactococcus lactis expressing outer membrane protein A of Shigella dysenteriae type‐1: evaluation of oral and intranasal route of administration
Author(s) -
Yagnik B.,
Sharma D.,
Padh H.,
Desai P.
Publication year - 2017
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.13353
Subject(s) - shigella dysenteriae , lactococcus lactis , microbiology and biotechnology , nasal administration , immunization , lactococcus , shigella flexneri , virology , biology , streptococcaceae , bacteria , medicine , antibiotics , antibody , immunology , lactic acid , escherichia coli , biochemistry , genetics , gene
Aims To evaluate the comparative immunogenic potential of food grade Lactococcus lactis expressing outer membrane protein A (OmpA) of Shigella dysenteriae type‐1 ( SD ‐1) when administered either orally or intranasally. Methods and Results OmpA of SD ‐1 was cloned and expressed first in Escherichia coli and then in L. lactis . Presence of recombinant gene was confirmed by restriction enzyme digestion and immunoblot analysis. Using immobilized metal affinity chromatography, OmpA was purified from recombinant E. coli BL 21 ( DE 3) and subcutaneously administered in BALB /c mice. Detection of OmpA‐specific IgG antibodies by enzyme‐linked immunosorbent assay ( ELISA ) confirmed the immunogenicity of OmpA. In order to establish r‐ L. lactis as a mucosal delivery vehicle, it was administered orally and nasally in BALB /c mice. Serum IgG and faecal IgA were assessed through ELISA to compare the relative potential of immunization routes and immunogenic potential of r‐ L. lactis . Immunization via the oral route proved superior to intranasal exposure. Conclusion Recombinant L. lactis expressing OmpA of SD ‐1 was found to be immunogenic. Oral administration of r‐ L. lactis elicited higher systemic and mucosal immune response when compared with the nasal route. Significance and Impact of the Study Using food grade recombinant L. lactis has implications in the development of a prophylactic against multidrug‐resistant Shigella, which can be used as a prospective vaccine candidate. Evaluating mucosal routes of immunization demonstrated that the oral route of administration elicited better immune response against OmpA of Shigella .