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In vitro activity of antifungals in combination with essential oils against the oomycete Pythium insidiosum
Author(s) -
Valente J.S.,
Fonseca A.O.S.,
Denardi L.B.,
Dal Ben V.S.,
Maia Filho F.S.,
Zambrano C.G.,
Braga C.Q.,
Alves S.H.,
Botton S.A.,
Brayer Pereira D.I.
Publication year - 2016
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.13234
Subject(s) - terbinafine , itraconazole , microbiology and biotechnology , minimum inhibitory concentration , biology , broth microdilution , essential oil , antifungal , antimicrobial , pharmacology , botany
Aims The aim of this study was to investigate the in vitro susceptibility of Pythium insidiosum to combinations of the antifungal drugs terbinafine or itraconazole with Melaleuca alternifolia , Mentha piperita and Origanum vulgare essential oils ( EO s). Methods and Results In vitro combinations of antifungal drugs with EOs were evaluated by checkerboard microdilution method against 20 Brazilian isolates of P. insidiosum . The tests were performed according to protocol M38‐A2, and the interpretation of each combination result was based on the values of the fractional inhibitory concentration index. The combinations of itraconazole with EOs presented prominent synergistic effects against P. insidiosum isolates, and no antagonism was observed with these combinations. However, the combinations of terbinafine with EOs resulted in indifferent or antagonistic effects. Conclusions The combination of plant‐derived bioactive compounds with antifungal drugs may be an alternative therapy for the control of infections caused by P. insidiosum . Studies of new therapeutic protocols involving these proposed combinations are needed. Significance and Impact of the Study The antimicrobial combinations using EO s with terbinafine or itraconazole can be an attractive therapeutic option for controlling P. insidiosum infections.