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Cholinesterase inhibitor (Altenuene) from an endophytic fungus Alternaria alternata : optimization, purification and characterization
Author(s) -
Bhagat J.,
Kaur A.,
Kaur R.,
Yadav A.K.,
Sharma V.,
Chadha B.S.
Publication year - 2016
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.13192
Subject(s) - alternaria alternata , biology , plant use of endophytic fungi in defense , biochemistry , catharanthus roseus , butyrylcholinesterase , cholinesterase , microbiology and biotechnology , acetylcholinesterase , botany , aché , pharmacology , enzyme
Aims The aim of this study was to screen endophytic fungi isolated from Vinca rosea for their potential to produce acetylcholinesterase (AChE) inhibitors. Method and Results Endophytic fungi isolated from V. rosea ( Catharanthus roseus ), were screened for AChE inhibitor production using Ellman's method. Maximum inhibition against AChE (78%) was observed in an isolate VS‐10, identified to be Alternaria alternata on morphological and molecular basis. The isolate also inhibited butyrylcholinesterase (73%). Significant increase (1·3 fold) was achieved after optimization of process parameters using one variable at time approach. The inhibitor was purified using chromatographic techniques. The structure elucidation of the inhibitor was carried out using spectroscopic techniques and was identified to be ‘altenuene’. The purified inhibitor possessed antioxidant potential as revealed by dot blot assay. The insecticidal potential of purified inhibitor was evaluated by feeding Spodoptora litura on diet amended with inhibitor. It evinced significant larval mortality. Conclusions Endophytic A. alternata can serve as a source of dual cholinesterase inhibitor ‘altenuene’ with significant antioxidant and insecticidal activity. This is the first report on acetylcholinestearse inhibitory activity of altenuene. Significance and Impact of the Study Alternaria alternata has the potential to produce a dual ChE inhibitor with antioxidant activity useful in the treatment of neurodegenerative disorders and in agriculture as biocontrol agent.

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