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Staphylococcus epidermidis biofilm on implant material is reduced by a covalently linked thiophenone
Author(s) -
Aamdal Scheie Anne,
Chamgordani Elahe Jafari,
Naemi AliOddin,
Hansen Finn Knut,
Benneche Tore
Publication year - 2016
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.13188
Subject(s) - biofilm , staphylococcus epidermidis , covalent bond , coating , implant , materials science , x ray photoelectron spectroscopy , chemistry , microbiology and biotechnology , chemical engineering , nanotechnology , bacteria , staphylococcus aureus , biology , medicine , organic chemistry , genetics , surgery , engineering
Aims The present aims were firstly to coat metal implant material with a quorum sensing inhibitory thiophenone molecule, and secondly to assess the inhibitory effect on Staphylococcus epidermidis biofilm accumulation on thiophenone‐coated coupons. Method and Results Thiophenone‐ and control‐coated metal coupons were prepared by silane hydrolysis and dip coating. The linking of thiophenone to the surface was confirmed by X‐ray photoelectron spectroscopy analyses. Biofilm by Staph. epidermidis, a frequent cause of implant‐associated infections, was allowed to form under flowing conditions for 48 h. The biofilm accumulations were significantly reduced on the thiophenone‐coated coupons. This was confirmed by confocal scanning microscopy. Conclusion This study showed for the first time how a synthetic thiophenone may be covalently linked to a stainless steel surface, and that biofilm accumulations on such surfaces are significantly reduced. Significance and Impact of the Study Functionalizing surfaces by covalent linking of thiophenones might open a wide array of applications. Thiophenone coating of medical implants represents a novel and promising approach to prevent implant‐associated infections.