Premium
Reactive oxygen species production, induced by atmospheric modification, alter conidial quality of Beauveria bassiana
Author(s) -
PérezGuzmán D.,
MontesinosMatías R.,
ArceCervantes O.,
GómezQuiroz L.E.,
Loera O.,
GarzaLópez P.M.
Publication year - 2016
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.13156
Subject(s) - beauveria bassiana , infectivity , bassiana , reactive oxygen species , conidium , bioassay , microbiology and biotechnology , biology , beauveria , horticulture , chemistry , food science , botany , biological pest control , biochemistry , ecology , virology , virus
Aim The aim of this study was to determine the relationship between reactive oxygen species ( ROS ) production and conidial infectivity in Beauveria bassiana . Methods and Results Beauveria bassiana Bb 882.5 was cultured in solid‐state culture ( SSC ) using rice under three oxygen conditions (21%, or pulses at 16 and 26%). Hydrophobicity was determined using exclusion phase assay. Bioassays with larvae or adults of Tenebrio molitor allowed the measurements of infectivity parameters. A fluorometric method was used for ROS quantification (superoxide and total peroxides). NADPH oxidase ( NOX ) activity was determined by specific inhibition. Conidial hydrophobicity decreased by O 2 pulses. Mortality of larvae was only achieved with conidia harvested from cultures under 21% O 2 ; whereas for adult insects, the infectivity parameters deteriorated in conidia obtained after pulses at 16 and 26% O 2 . At day 7, ROS production increased after 16 and 26% O 2 treatments. NOX activity induced ROS production at early stages of the culture. Conclusion Modification of atmospheric oxygen increases ROS production, reducing conidial quality and infectivity. Significance and Impact of the Study This is the first study in which conidial infectivity and ROS production in B. bassiana has been related, enhancing the knowledge of the effect of O 2 pulses in B. bassiana .