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Characterization of Staphylococcus aureus faecal isolates associated with food‐borne disease in Korea
Author(s) -
Shin E.,
Hong H.,
Park J.,
Oh Y.,
Jung J.,
Lee Y.
Publication year - 2016
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.13133
Subject(s) - staphylococcus aureus , microbiology and biotechnology , biology , bacteria , genetics
Aims To characterize Staphylococcus aureus faecal isolates from people suspected to be infected with food poisoning by using antimicrobial susceptibility testing and molecular techniques. Methods and Results A total of 340 Staph. aureus isolates from 6226 people suspected to be infected with food poisoning were identified and characterized by biochemical methods, antimicrobial susceptibility testing and PCR . Samples were obtained from January 2006 to December 2008 from the National Notifiable Diseases Surveillance System at the Research Institute of Public Health and Environment in Seoul Metropolitan, Korea. All strains carried at least one of the eight staphylococcal enterotoxin ( se ) genes tested and a total of 27 se profiles were produced; the most frequent se profile was seg‐sei and the next was sea . Among the total isolates, 36 methicillin‐resistant Staphylococcus aureus ( MRSA s) isolates were further analysed by multilocus sequence typing ( MLST ), Staphylococcal cassette chromosome mec ( SCC mec ) typing, pulsed‐field gel electrophoresis ( PFGE ) and PCR detection for pvl . ST 72‐ SCC mec type IV was the most predominant clone (27 isolates, 75%) followed by ST 1‐ SCC mec type IV (five isolates, 13·8%), ST 20‐ SCC mec type IV (one isolate, 2·8%), ST 493‐ SCC mec type IV (one isolate, 2·8%), ST 903‐ SCC mec type IV (one isolate, 2·8%) and ST 5‐ SCC mec type II (one isolate, 2·8%). By PFGE typing, MRSA s isolated during the same period were grouped together although they were isolated from different regions. None of MRSA s had PVL gene and nine MRSA s were multidrug resistant. Conclusions Analysis of MRSA s by MLST , SCC mec typing, PFGE and pvl detection showed that the majority of strain associated with food‐borne diseases belonged to a Korean community‐acquired ( CA ) MRSA clone with ST 72‐ SCC mec type IV ‐ PVL negative‐ SEG / SEI and its variations while one strain was hospital‐acquired ( HA ) MRSA . Significance and Impact of the Study CA ‐ MRSA clone which possessed ST 72‐ SCC mec type IV ‐ PVL negative‐ SEG / SEI was spread most commonly among MRSA s that were associated with food‐borne diseases. This is the first report of ST 903 strain in Korea.