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Optimization of a protective medium for freeze‐dried Pichia membranifaciens and application of this biocontrol agent on citrus fruit
Author(s) -
Niu X.,
Deng L.,
Zhou Y.,
Wang W.,
Yao S.,
Zeng K.
Publication year - 2016
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.13129
Subject(s) - penicillium italicum , penicillium digitatum , biology , biological pest control , food science , yeast , botany , horticulture , fungicide , biochemistry
Aims To optimize a protective medium for freeze‐dried Pichia membranifaciens and to evaluate biocontrol efficacies of agents against blue and green mould and anthracnose in citrus fruit. Methods and Results Based on the screening assays of saccharides and antioxidants, response surface methodology was used to optimize sucrose, sodium glutamate and skim milk to improve viability of freeze‐dried Pi. membranifaciens . Biocontrol assays were conducted between fresh and freeze‐dried Pi. membranifaciens against Penicillium italicum , Penicillium digitatum and Colletotrichum gloeosporioides in citrus fruit. Solving the regression equation indicated that the optimal protective medium was 6·06% (w/v) sucrose combined with 3·40% (w/v) sodium glutamate and 5·43% (w/v) skim milk. Pi. membranifaciens freeze‐dried in the optimal protective medium showed 76·80% viability, and retained biocontrol efficacy against Pe. italicum , Pe. digitatum and Co. gloeosporioides in citrus fruit. Conclusions The optimal protective medium showed more effective protective properties than each of the three protectants used alone. The viability of freeze‐dried Pi. membranifaciens finally reached 76·80%. Meanwhile, the biocontrol efficacies showed no significant difference between fresh and freeze‐dried yeast against Pe. italicum , Pe. digitatum and Co. gloeosporioides in citrus fruit. Significance and Impact of the Study The results showed the potential value of Pi. membranifaciens CICC 32259 for commercialization.