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Biodiversity and technological‐functional potential of lactic acid bacteria isolated from spontaneously fermented quinoa sourdoughs
Author(s) -
Ruiz Rodríguez L.,
Vera Pingitore E.,
Rollan G.,
Cocconcelli P. S.,
Fontana C.,
Saavedra L.,
Vignolo G.,
Hebert E. M.
Publication year - 2016
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.13104
Subject(s) - biology , lactobacillus plantarum , fermentation , lactic acid , leuconostoc mesenteroides , lactobacillus brevis , food science , starter , bacteria , lactobacillus , temperature gradient gel electrophoresis , leuconostoc , fermentation in food processing , microbiology and biotechnology , 16s ribosomal rna , genetics
Aims To analyse lactic acid bacteria ( LAB ) diversity and technological‐functional and safety properties of strains present during spontaneous fermented quinoa sourdoughs. Methods and Results Fermentation was performed by daily backslopping at 30°C for 10 days. Autochthonous LAB microbiota was monitored by a biphasic approach combining random amplified polymorphic DNA ( RAPD )‐ PCR and rRNA gene sequencing with PCR ‐denaturing gradient gel electrophoresis ( DGGE ) analysis. Identification and intraspecies differentiation allowed to group isolates within nine LAB species belonging to four genera. A succession of LAB species occurred during 10‐days backslopping; Lactobacillus plantarum and Lactobacillus brevis were detected as dominant species in the consortium. The characterization of 15 representative LAB strains was performed based on the acidifying capacity, starch and protein hydrolysis, γ ‐aminobutyric acid and exopolysaccharides production, antimicrobial activity and antibiotic resistance. Conclusion Strains characterization led to the selection of Lact. plantarum CRL 1905 and Leuconostoc mesenteroides CRL 1907 as candidates to be assayed as functional starter culture for the gluten‐free ( GF ) quinoa fermented products. Significance and Impact of the Study Results on native LAB microbiota present during quinoa sourdough fermentation will allow the selection of strains with appropriate technological properties to be used as a novel functional starter culture for GF ‐fermented products.