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Study of silver nanoparticles synthesized by acidophilic strain of Actinobacteria isolated from the of Picea sitchensis forest soil
Author(s) -
RaileanPlugaru V.,
Pomastowski P.,
Wypij M.,
SzultkaMlynska M.,
Rafinska K.,
Golinska P.,
Dahm H.,
Buszewski B.
Publication year - 2016
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.13093
Subject(s) - proteus mirabilis , microbiology and biotechnology , antimicrobial , escherichia coli , chemistry , bacillus subtilis , klebsiella pneumoniae , silver nanoparticle , bacteria , biology , nanoparticle , materials science , biochemistry , nanotechnology , genetics , gene
Aims In the present work the acidophilic actinobacteria strain was used as a novel reducing agent for the cheap, green and single‐step synthesis of nanostructure silver particles. Structural, morphological and optical properties of the synthesized nanoparticles have been characterized by spectroscopy, dynamic light scattering and electron microscopy approach. The antimicrobial activity of silver nanoparticles against clinical strains such as Staphylococcus aureus , Bacillus subtilis , Escherichia coli , Pseudomonas aeruginosa , Klebsiella pneumoniae , Proteus mirabilis and Salmonella infantis alone and in combination with antibiotics were studied. Methods and Results The crystalline and stable biosynthesized silver nanoparticles ranged in size from 4 to 45 nm and were mostly spherical in shape being characterized evolving several analytical techniques. The bioAg NP s inhibited growth of most bacterial strains. The highest antimicrobial activity was observed against Ps. aeruginosa (10 mm), followed by Staph. aureus , B. subtilis and Pr. mirabilis (all 8 mm). The lower activity was noticed for E. coli and Kl. pneumoniae (6 and 2 mm, respectively). Moreover, the synergistic effect of bio(Ag NP s) with various commercially available antibiotics was also evaluated. The most significant results were observed for bio(Ag NP s) combined with tetracycline, kanamycin, ampicillin and neomycin, followed by streptomycin and gentamycin against E. coli , Salm. infantis and Kl. pneumoniae . The most resistant bacteria to commercial antibiotics was Pr. mirabilis . Conclusion The Streptacidiphilus sp. strain CGG 11n isolated from acidic soil can be used to efficiently synthesize the bioactive nanoparticles using inexpensive substances in an eco‐friendly and nontoxic manner. The present work provides helpful insight into the development of new antimicrobial agents with the synergistic enhancement of the antibacterial mechanism against pathogenic micro‐organisms. Significance and Impact of the Study The synthesized silver bionanoparticles from Streptacidiphilus sp. strain CGG 11n possess potent inhibitory effect that offers valuable contribution to pharmaceutical associations.