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Optimization of large‐ring cyclodextrin production from starch by amylomaltase from Corynebacterium glutamicum and effect of organic solvent on product size
Author(s) -
Vongpichayapaiboon T.,
Pongsawasdi P.,
Krusong K.
Publication year - 2016
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.13087
Subject(s) - starch , chemistry , yield (engineering) , incubation , pullulanase , chromatography , corynebacterium glutamicum , dimethyl sulfoxide , biochemistry , food science , organic chemistry , materials science , metallurgy , gene
Aims To increase yield of starch conversion to large‐ring cyclodextrins ( LR ‐ CD s) by amylomaltase from Corynebacterium glutamicum ( Cg AM ). Methods and Results In this work, LR ‐ CD s produced from pea, tapioca, corn, potato, rice and glutinous‐rice starch by the recombinant Cg AM were analysed by High‐Performance Anion‐Exchange Chromatography Using Pulsed Amperometric Detection ( HPAEC ‐ PAD ). Among these, pea starch gave the highest yield of LR ‐ CD s. Pretreatment of pea starch with isoamylase prior to incubation with Cg AM resulted in the increase in LR ‐ CD products by 20%. Surprisingly, Cg AM converted starch into LR ‐ CD s within a wide pH range ( pH 5·5–9·0). LR ‐ CD syntheses at alkaline pH or at a long incubation time favoured low‐degree of polymerization ( DP ) products ( CD 22– CD 32). Addition of 5–15% dimethyl sulfoxide ( DMSO ) promoted the synthesis of medium‐ DP species ( CD 33– CD 43) by 10–25%. Conclusions Pretreatment of pea starch with isoamylase could enhance the yield of LR ‐ CD s. The ratio of LR ‐ CD products depends on pH , incubation time and addition of organic solvents such as DMSO . Significance and Impact of the Study LR ‐ CD yield can be increased by thorough optimization of starch types, starch concentrations, enzyme activities, pH and incubation times. This study is the first report of the effect of organic solvents on LR ‐ CD production by amylomaltase.