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Diversity and variation in antimicrobial susceptibility patterns over time in Mycoplasma agalactiae isolates collected from sheep and goats in France
Author(s) -
Poumarat F.,
GautierBouchardon A.V.,
Bergonier D.,
Gay E.,
Tardy F.
Publication year - 2016
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.13083
Subject(s) - biology , genetic diversity , mycoplasma , microbiology and biotechnology , antibiotics , antimicrobial , host (biology) , genetic variation , pathogen , herd , genetics , gene , ecology , population , demography , sociology
Aims Mycoplasma agalactiae is responsible for Contagious Agalactia, a severe syndrome affecting small ruminants worldwide and resulting in significant economic losses in countries with an important dairy industry. The aim of this study was to examine the antimicrobial susceptibility patterns of M. agalactiae isolates in France, their evolution over the last 25 years and their relationships with the genetic diversity of isolates and their origin (geographical and animal host). Methods and Results Susceptibility patterns were determined by measuring minimal inhibitory concentrations ( MIC s) of several antimicrobials used against mycoplasmas. Caprine M. agalactiae strains showed increased MIC s over time for most of the antimicrobials tested, except fluoroquinolones. This susceptibility loss was homogeneous despite the considerable genetic and geographical heterogeneity of the isolates. In contrast, all the ovine isolates originating from a single clone and the same region showed increased MIC s only to some macrolides. Conclusions MIC s have evolved differently depending on the origin of the isolates but the overall loss in susceptibility has remained far more moderate than that of Mycoplasma bovis , a cattle pathogen closely related to M. agalactiae . Significance and Impact of the Study Several hypotheses are proposed to explain the differences in susceptibility patterns, such as local, specific, nonmycoplasma‐targeting antibiotic treatments and the genetic background of isolates in connection with their animal host.