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In vitro antiviral activity of Lactobacillus casei and Bifidobacterium adolescentis against rotavirus infection monitored by NSP 4 protein production
Author(s) -
Olaya Galán N.N.,
Ulloa Rubiano J.C.,
Velez Reyes F.A.,
Fernandez Duarte K.P.,
Salas Cárdenas S.P.,
Gutierrez Fernandez M.F.
Publication year - 2016
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.13069
Subject(s) - lactobacillus casei , probiotic , rotavirus , microbiology and biotechnology , in vitro , biology , bifidobacterium , flow cytometry , liberation , intracellular , lactobacillus , bifidobacterium breve , biochemistry , virology , bacteria , virus , genetics , fermentation
Aims The aim of this study was to determine the antiviral activity of four probiotic metabolites ( Lactobacillus and Bifidobacetrium species) against rotavirus in vitro infection monitored by the NSP 4 protein production and Ca 2+ release. Methods and Results The antiviral effect of the metabolites was performed due a comparison between a blocking model and an intracelullar model on MA 104 cells, with the response of NSP 4 production and Ca 2+ liberation measured by flow cytometry. Significant results were obtained with the metabolites of Lactobacillus casei, and Bifidobacterium adolescentis in the reduction of the protein production ( P = 0·04 and P = 0·014) and Ca 2+ liberation ( P = 0·094 and P = 0·020) in the intracellular model, which suggests a successful antiviral activity against RV infection. Conclusions This study demonstrates that probiotic metabolites were able to interfere with the final amount of intracellular NSP 4 protein and a successful Ca 2+ regulation, which suggests a new approach to the mechanism exerted by probiotics against the rotavirus infection. Significance and Impact of the Study A novel anti‐rotaviral effect exerted by probiotic metabolites monitored by the NSP 4 protein during the RV in vitro infection and the effect on the Ca 2+ release is reported; suggesting a reduction on the impact of the infection by decreasing the damage of the cells preventing the electrolyte loss.