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Nitric oxide‐releasing polyacrylonitrile disperses biofilms formed by wound‐relevant pathogenic bacteria
Author(s) -
Craven M.,
Kasper S.H.,
Canfield M.J.,
DiazMorales R.R.,
Hrabie J.A.,
Cady N.C.,
Strickland A.D.
Publication year - 2016
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.13059
Subject(s) - polyacrylonitrile , biofilm , bacteria , microbiology and biotechnology , nitric oxide , pathogenic bacteria , chemistry , biology , organic chemistry , polymer , genetics
Aims To test the antimicrobial and antibiofilm properties of a nitric oxide ( NO )‐releasing polymer against wound‐relevant bacterial pathogens. Methods and Results Using a variety of 96‐well plate assay systems that include standard well plates and the minimum biofilm eradication concentration biofilm assay well plate, a NO ‐releasing polymer based on (poly)acrylonitrile ( PAN / NO ) was studied for antimicrobial and antibiofilm activity against the common wound pathogens Pseudomonas aeruginosa ( PAO 1), Staphylococcus aureus (Mu50) and Enterococcus faecalis (V583). The polymer was capable of dispersing single‐species biofilms of Ps. aeruginosa as well as a more clinically relevant multispecies biofilm that incorporates Ps. aeruginosa along with Staph. aureus and Ent. faecalis . PAN / NO also synergistically enhanced the susceptibility of the multispecies biofilms to the common broad‐spectrum antibiotic, ciprofloxacin. Multiple in vitro biocompatibility assays show that PAN / NO has limited potential for mammalian cytotoxicity. Conclusion This study demonstrates the feasibility of utilizing the NO ‐releasing polymer, PAN / NO , to manage biofilms formed by wound‐relevant pathogens, and provides proof‐of‐concept for use of this NO ‐releasing polymer platform across multiple disciplines where bacterial biofilms pose significant problems. Significance and Impact of Study In the clinical sector, bacterial biofilms represent a substantial treatment challenge for health care professionals and are widely recognized as a key factor in prolonging patient morbidity. This study highlights the potential role for the ubiquitous signalling molecule nitric oxide ( NO ) as an antibiofilm therapy.