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Inactivation of Dengue and Yellow Fever viruses by heme, cobalt‐protoporphyrin IX and tin‐protoporphyrin IX
Author(s) -
AssunçãoMiranda I.,
CruzOliveira C.,
Neris R.L.S.,
Figueiredo C.M.,
Pereira L.P.S.,
Rodrigues D.,
Araujo D.F.F.,
Da Poian A.T.,
Bozza M.T.
Publication year - 2016
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.13038
Subject(s) - virology , heme , hmox1 , viral replication , dengue virus , protoporphyrin , biology , protoporphyrin ix , dengue fever , virus , porphyrin , microbiology and biotechnology , heme oxygenase , chemistry , biochemistry , photodynamic therapy , enzyme , organic chemistry
Aims To investigate the effect of heme, cobalt‐protoporphyrin IX and tin‐protoporphyrin IX (Co PPIX and Sn PPIX ), macrocyclic structures composed by a tetrapyrrole ring with a central metallic ion, on Dengue Virus ( DENV ) and Yellow Fever Virus ( YFV ) infection. Methods and Results Treatment of HepG2 cells with heme, Co PPIX and Sn PPIX after DENV infection reduced infectious particles without affecting viral RNA contents in infected cells. The reduction of viral load occurs only with the direct contact of DENV with porphyrins, suggesting a direct effect on viral particles. Previously incubation of DENV and YFV with heme, Co PPIX and Sn PPIX resulted in viral particles inactivation in a dose‐dependent manner. Biliverdin, a noncyclical porphyrin, was unable to inactivate the viruses tested. Infection of HepG2 cells with porphyrin‐pretreated DENV 2 results in a reduced or abolished viral protein synthesis, RNA replication and cell death. Treatment of HepG2 or THP ‐1 cell lineage with heme or Co PPIX after DENV infection with a very low MOI resulted in a decreased DENV replication and protection from death. Conclusions Heme, Co PPIX and Sn PPIX possess a marked ability to inactivate DENV and YFV , impairing its ability to infect and induce cytopathic effects on target cells. Significance and Impact of the Study These results open the possibility of therapeutic application of porphyrins or their use as models to design new antiviral drugs against DENV and YFV .