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Metabolic flux ratio analysis and cell staining suggest the existence of C 4 photosynthesis in Phaeodactylum tricornutum
Author(s) -
Huang A.,
Liu L.,
Zhao P.,
Yang C.,
Wang G.C.
Publication year - 2016
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.13020
Subject(s) - phaeodactylum tricornutum , photosynthesis , organelle , metabolic flux analysis , flux (metallurgy) , biology , carbon fixation , biochemistry , crassulacean acid metabolism , biophysics , metabolism , botany , diatom , chemistry , organic chemistry
Aims Mechanisms for carbon fixation via photosynthesis in the diatom Phaeodactylum tricornutum Bohlin were studied recently but there remains a long‐standing debate concerning the occurrence of C 4 photosynthesis in this species. A thorough investigation of carbon metabolism and the evidence for C 4 photosynthesis based on organelle partitioning was needed. Methods and Results In this study, we identified the flux ratios between C 3 and C 4 compounds in P. tricornutum using 13 C‐labelling metabolic flux ratio analysis, and stained cells with various cell‐permeant fluorescent probes to investigate the likely organelle partitioning required for single‐cell C 4 photosynthesis. Metabolic flux ratio analysis indicated the C 3 /C 4 exchange ratios were high. Cell staining indicated organelle partitioning required for single‐cell C 4 photosynthesis might exist in P. tricornutum . Conclusion The results of 13 C‐labelling metabolic flux ratio analysis and cell staining suggest single‐cell C 4 photosynthesis exists in P. tricornutum . Significance and Impact of the Study This study provides insights into photosynthesis patterns of P. tricornutum and the evidence for C 4 photosynthesis based on 13 C‐labelling metabolic flux ratio analysis and organelle partitioning.