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Optimizing the production of the biosurfactant lichenysin and its application in biofilm control
Author(s) -
CoronelLeón J.,
Marqués A.M.,
Bastida J.,
Manresa A.
Publication year - 2016
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12992
Subject(s) - biofilm , bacillus licheniformis , food science , microbiology and biotechnology , response surface methodology , chemistry , staphylococcus aureus , bacteria , biology , bacillus subtilis , chromatography , genetics
Aims Apply response surface methodology ( RSM ) to develop and optimize an economical medium for lichenysin production, which is a surfactant produced by Bacillus licheniformis and evaluate the application of lichenysin in the prevention and disruption of pathogenic micro‐organism biofilm that creates health problems in the food industry and hospitals. Results An economical medium containing molasses was optimized to enhance lichenysin production by RSM . A production of 3·2 g l −1 of lichenysin was achieved with an optimum medium containing 107·82 g l −1 of molasses, 6·47 g l −1 of Na NO 3 and 9·7 g l −1 of K 2 HPO 4 / KH 2 PO 4 , in which molasses and phosphate salts had a significant effect on biosurfactant production. Lichenysin was effectively applied in a surface pre‐treatment to avoid microbial biofilm development of methicillin‐resistant Staphylococcus aureus ( MRSA ) (68·73%) and Candida albicans (74·35%), with ED 50 values of 8·3 and 17·2 μ g ml −1 respectively. It was also very efficient in a surface post‐treatment to remove biofilms of MRSA (55·74%) and Yersinia enterocolitica (51·51%), with an ED 50 of 2·79 and 4·09 μ g ml −1 respectively. Conclusions Lichenysin was found to have notable anti‐adhesion activity, being able to prevent and eliminate the biofilm formation by pathogenic strains associated with foodborne illness. This new medium resulted in a four‐fold increase in production compared with the nonoptimized medium. Significance and Impact of the Study Molasses can be regarded as a useful resource for biotechnological applications, such as the production of lichenysin. The use of agro‐industrial substrates has an important role in the sustainable and competitive development of several industrial sectors, as well as in industrial residues management. Additionally, lichenysin is particularly effective in preventing biofilm formation by strains problematic for the food industry and in the hospital environment. Lichenysin also efficiently disrupts biofilm.