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Fermentative profile and bacterial diversity of corn silages inoculated with new tropical lactic acid bacteria
Author(s) -
Santos A.O.,
Ávila C.L.S.,
Pinto J.C.,
Carvalho B.F.,
Dias D.R.,
Schwan R.F.
Publication year - 2016
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12980
Subject(s) - silage , microbial inoculant , lactobacillus buchneri , biology , food science , lactic acid , lactobacillus plantarum , fermentation , lactobacillaceae , bacteria , microbiology and biotechnology , inoculation , lactobacillus , horticulture , genetics
Abstract Aims This study aimed to evaluate the effects of inoculation of strains of lactic acid bacteria ( LAB ) isolated from sugarcane grown in a Brazil on the quality of corn silage. Methods and Results Three strains of Lactobacillus buchneri ( UFLA SLM 11, UFLA SLM 103 and UFLA SLM 108), five strains of Lactobacillus plantarum ( UFLA SLM 08, UFLA SLM 41, UFLA SLM 45, UFLA SLM 46 and UFLA SLM 105), and one strain of Leuconostoc mesenteroides ( UFLA SLM 06) were evaluated at 0, 10, 30, 60 and 90 day after inoculating corn forage. The inoculation of the LAB strains did not influence the chemical composition of the silage, but pH , acetic acid and 1,2‐propanediol were affected by treatment. The silages inoculated with UFLA SLM 11 and SLM 108 contained the lowest yeast and filamentous fungi counts during fermentation. Bacteria belonging to the Enterobacteriaceae family, Clostridium genus were detected in the silages inoculated with Lact. buchneri UFLA SLM 11, 103 and 108, as shown by DGGE analysis. Silages inoculated with Lact. buchneri UFLA SLM 11 showed higher aerobic stability. Conclusions The Lact. buchneri UFLA SLM 11 strain was considered promising as a starter culture or inoculant for corn silages. Significance and Impact of the Study The selection of microbial inoculants for each crop promotes improvement of silage quality. Studies on the chemical and microbiological characteristics of silage provide useful information for improving ensiling techniques.