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Efficacy of Tolypocladium cylindrosporum against Aedes aegypti eggs, larvae and adults
Author(s) -
Rocha L.F.N.,
Sousa N.A.,
Rodrigues J.,
Catão A.M.L.,
Marques C.S.,
Fernandes É.K.K.,
Luz C.
Publication year - 2015
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12945
Subject(s) - aedes aegypti , larva , biology , conidium , instar , veterinary medicine , biological pest control , vector (molecular biology) , pupa , toxicology , botany , medicine , biochemistry , gene , recombinant dna
Aims The aim of this study was to test the activity of seven Tolypocladium cylindrosporum isolates against different developmental stages of Aedes aegypti . Methods and Results Ovicidal activity (≤30% of eclosion; at a 84% eclosion of control eggs) and lowest cumulative emergence of adults (≤30%; at a 66·3% control emergence) originating from eclosing larvae was found 15 and 30 days, respectively, after applying conidia of ARSEF 962 or 1580 onto the eggs. All isolates induced larval mortality when third‐instar larvae were treated with conidia (≤5% control mortality); the lowest lethal concentration ( LC 50 ) at 5 days after treatment was found with ARSEF 1580 (9·2 × 10 5 conidia ml −1 ), and the shortest lethal time ( LT 50 ) with ARSEF 2912 (2·8 days at 3·3 × 10 6 conidia ml −1 ). The pathogenicity of T. cylindrosporum to A. aegypti adults is corroborated. ARSEF 1027, 1580 and 2912 induced the highest cumulative mortality in adults (≥90%) 15 days after application (≤5% control mortality). Conclusions Findings emphasized an isolate‐ and developmental stage‐related virulence of T. cylindrosporum . Significance and Impact of the Study ARSEF 1580, in the group of tested isolates, is the most promising isolate for use against terrestrial eggs, aquatic larvae and adults of this important vector.