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Oral administration of L actobacillus casei variety rhamnosus partially alleviates TMA ‐induced atopic dermatitis in mice through improving intestinal microbiota
Author(s) -
Yeom M.,
Sur B.J.,
Park J.,
Cho S.G.,
Lee B.,
Kim S.T.,
Kim K.S.,
Lee H.,
Hahm D.H.
Publication year - 2015
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12844
Subject(s) - lactobacillus casei , bacteroides , atopic dermatitis , probiotic , bacteroides fragilis , lactobacillus rhamnosus , population , microbiology and biotechnology , immunology , bifidobacterium , biology , gut flora , lactobacillus , medicine , bacteria , antibiotics , genetics , environmental health
Aims The purpose of this study was to investigate the effect of Lactobacillus casei variety rhamnosus ( LCR 35) on Atopic dermatitis ( AD )‐like symptoms in mice. Methods and Results AD ‐like skin lesions in BALB /C mice were induced by sensitization and subsequent repeated challenges with trimellitic anhydride ( TMA ) for 10 days. LCR 35 was orally administered to the mice once daily throughout the study. In the TMA ‐induced AD model, orally administered LCR 35 suppressed significantly irritant‐related scratching behaviour and skin dehydration as well as apparent severity of AD . LCR 35 also significantly decreased serum levels of IgE and IL ‐4, but not IFN ‐ γ , implying the restoration of TMA ‐induced disruption of Th1/Th2 balance. Quantitative real‐time PCR targeting hypervariable regions of 16S rDNA gene of faecal microbiota indicated that the LCR 35 treatment increased the population of Bifidobacterium , Lactobacilli , Enterococcus and Bacteroides fragilis group, but decreased those of Clostridium coccoides group. Conclusions LCR 35 has the ability to suppress the development of AD in mice, possibly through the modulation of Th1/Th2 balance and gut microbiota. Significance and Impact of the Study LCR 35 has a strong potential as a probiotic for preventing AD .