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Pseudomonas moraviensis subsp. stanleyae, a bacterial endophyte of hyperaccumulator S tanleya pinnata , is capable of efficient selenite reduction to elemental selenium under aerobic conditions
Author(s) -
Staicu L.C.,
Ackerson C.J.,
Cornelis P.,
Ye L.,
Berendsen R.L.,
Hunter W.J.,
Noblitt S.D.,
Henry C.S.,
Cappa J.J.,
Montenieri R.L.,
Wong A.O.,
Musilova L.,
Surade Jong M.,
van Hullebusch E.D.,
Lens P.N.L.,
Reynolds R.J.B.,
PilonSmits E.A.H.
Publication year - 2015
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12842
Subject(s) - selenium , selenate , pseudomonas , biology , stenotrophomonas , hyperaccumulator , botany , bioremediation , endophyte , bacteria , food science , chemistry , phytoremediation , ecology , soil water , organic chemistry , genetics
Aims To identify bacteria with high selenium tolerance and reduction capacity for bioremediation of wastewater and nanoselenium particle production. Methods and Results A bacterial endophyte was isolated from the selenium hyperaccumulator Stanleya pinnata (Brassicaceae) growing on seleniferous soils in Colorado, USA . Based on fatty acid methyl ester analysis and multi‐locus sequence analysis ( MLSA ) using 16S rRNA , gyrB , rpoB and rpoD genes, the isolate was identified as a subspecies of Pseudomonas moraviensis (97·3% nucleotide identity) and named P. moraviensis stanleyae. The isolate exhibited extreme tolerance to SeO 3 2− (up to 120 mmol l −1 ) and SeO 4 2− (>150 mmol l −1 ). Selenium oxyanion removal from growth medium was measured by microchip capillary electrophoresis (detection limit 95 nmol l −1 for SeO 3 2− and 13 nmol l −1 for SeO 4 2− ). Within 48 h, P. moraviensis stanleyae aerobically reduced SeO 3 2− to red Se(0) from 10 mmol l −1 to below the detection limit (removal rate 0·27 mmol h −1 at 30°C); anaerobic SeO 3 2− removal was slower. No SeO 4 2− removal was observed. Pseudomonas moraviensis stanleyae stimulated the growth of crop species Brassica juncea by 70% with no significant effect on Se accumulation. Conclusions Pseudomonas moraviensis stanleyae can tolerate extreme levels of selenate and selenite and can deplete high levels of selenite under aerobic and anaerobic conditions. Significance and Impact of the Study Pseudomonas moraviensis subsp. stanleyae may be useful for stimulating plant growth and for the treatment of Se‐laden wastewater.