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Proteomic analysis reveals the mechanisms of M ycena dendrobii promoting transplantation survival and growth of tissue culture seedlings of D endrobium officinale
Author(s) -
Xu X.B.,
Ma X.Y.,
Lei H.H.,
Song H.M.,
Ying Q.C.,
Xu M.J.,
Liu S.B.,
Wang H.Z.
Publication year - 2015
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12781
Subject(s) - biology , seedling , tissue culture , botany , dendrobium , in vitro , biochemistry
Abstract Aims D endrobium officinale is an important traditional C hinese medicinal herb. Its seedlings generally show low survival and growth when transferred from in vitro tissue culture to a greenhouse or field environment. In this study, the effect of M ycena dendrobii on the survival and growth of D. officinale tissue culture seedlings and the mechanisms involved was explored. Methods and Results Mycena dendrobii were applied underneath the roots of D. officinale tissue culture seedlings. The seedling survival and growth were analysed. The root proteins induced by M. dendrobii were identified using two‐dimensional (2‐D) electrophoresis and matrix‐assisted laser desorption/ionization time‐of‐flight MS ( MALDI ‐ TOF ‐ MS ). Mycena dendrobii treatment significantly enhanced survival and growth of D. officinale seedlings. Forty‐one proteins induced by M. dendrobii were identified. Among them, 10 were involved in defence and stress response, two were involved in the formation of root or mycorrhizae, and three were related to the biosynthesis of bioactive constituents. Conclusions These results suggest that enhancing stress tolerance and promoting new root formation induced by M. dendrobii may improve the survival and growth of D. officinale tissue culture seedlings. Significance and Impact of the Study This study provides a foundation for future use of M. dendrobii in the large‐scale cultivation of Dendrobiums .