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In vitro evaluation of the acquisition of resistance, antifungal activity and synergism of B razilian red propolis with antifungal drugs on C andida spp.
Author(s) -
Pippi B.,
Lana A.J.D.,
Moraes R.C.,
Güez C.M.,
Machado M.,
Oliveira L.F.S.,
Lino von Poser G.,
Fuentefria A.M.
Publication year - 2015
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12746
Subject(s) - fluconazole , microbiology and biotechnology , anidulafungin , biology , candida glabrata , candida parapsilosis , propolis , in vitro , antifungal , traditional medicine , medicine , botany , biochemistry , micafungin
Aims To evaluate the ability of Candida parapsilosis and Candida glabrata to develop phenotypic resistance to a benzophenone enriched fraction obtained from Brazilian red propolis ( BZP ‐ BRP ) as compared to fluconazole (FLC). To investigate possible synergy between BZP ‐ BRP and FLC and anidulafungin (AND). Methods and Results To analyse the development of resistance, isolates susceptible to these antifungals were cultured in increasing concentrations of FLC and BZP ‐ BRP . The increase in FLC minimum inhibitory concentration for all isolates was evident and the majority developed resistance, whereas none isolated became less susceptible to BZP ‐ BRP . Synergism was investigated by checkerboard method. BZP ‐ BRP demonstrated synergy with FLC and indifference with AND for most isolates. Conclusions In conclusion, the synergism observed with FLC suggests that BZP ‐ BRP could be a possible therapeutic strategy for the treatment of infections related to FLC‐resistant Candida sp. Significance and Impact of the Study The indiscriminate use of antifungals results in the emergence of drug‐resistant strains among previously susceptible populations. BZP ‐ BRP can become an alternative for the treatment of persistent infections caused by Candida sp.