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Isolation of lactic acid bacteria from pao cai, a Chinese traditional fermented vegetable, with inhibitory activity against Salmonella associated with fresh‐cut apple, using a modelling study
Author(s) -
Luo W.,
Chen M.,
Chen A.,
Dong W.,
Hou X.,
Pu B.
Publication year - 2015
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12741
Subject(s) - salmonella , bacteria , food science , lactic acid , biology , fermentation , lactobacillus plantarum , inoculation , antibacterial activity , microbiology and biotechnology , horticulture , genetics
Aims To isolate lactic acid bacteria (LAB) from pao cai, a Chinese traditional fermented vegetable, with outstanding inhibitory activity against S almonella inoculated on fresh‐cut apple, using a modelling method. Methods and Results Four kinds of pao cai were selected. A total of 122 isolates exhibited typical LAB characteristics: Gram‐positive and catalase negative, among which 104 (85·24%) colonies showed antibacterial activity against S almonella by the well diffusion assay. Four colonies showing maximum antibacterial radius against S almonella were selected to co‐inoculate with S almonella on fresh‐cut apple and stored at 10°C, further identified as three strains of L actobacillus plantarum and one strain of L actobacillus brevis by 16s rRNA gene sequence analysis. The modified Gompertz model was employed to analyse the growth of the micro‐organisms on apple wedges. Two of the four selected strains showed antagonistic activity against S almonella on fresh‐cut apple, one of which, RD1, exhibited best inhibitory activity ( S almonella were greatly inhibited when co‐inoculated with RD1 at 10°C at 168 h). No deterioration in odour or appearance of the apple piece was observed by the triangle test when fresh‐cut apple was inoculated with RD1. Conclusions The mathematical modelling method is essential to select LAB with outstanding inhibitory activity against S almonella associated with fresh‐cut apple. LAB RD1 holds promise for the preservation of fresh‐cut apple. Significance and Impact of the Study This study provided a new method on fresh‐cut product preservation. Besides, to make the LAB isolating procedure a more correct one, this study first added the mathematical modelling method to the isolating procedure.