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Common occurrence of G allid herpesvirus‐2 with reticuloendotheliosis virus in chickens caused by possible contamination of vaccine stocks
Author(s) -
Woźniakowski G.,
Mamczur A.,
SamorekSalamonowicz E.
Publication year - 2015
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12734
Subject(s) - reticuloendotheliosis virus , biology , flock , bivalent (engine) , virology , virus , contamination , veterinary medicine , medicine , ecology , chemistry , organic chemistry , metal
Aims The aim of this study was to investigate the common occurrence of reticuloendotheliosis virus ( REV ) among Gallid herpesvirus 2 (Ga HV ‐2) infected chickens. The possible cause of this co‐occurrence may be linked to contaminated vaccine stocks, which were also examined. Methods and Results The study was conducted on 25 field isolates of Ga HV ‐2 collected between 2007 and 2013 from vaccinated chickens. Additionally, 10 commercial Marek's Disease vaccine stocks manufactured between 1993 and 2013, comprising of FC 126 HVT , CVI 988/Rispens and bivalent HVT + Rispens vaccines were examined. Chicken isolates were collected from the liver. Due to difficulties in differentiation between Ga HV ‐2 and REV , by observation of clinical signs or lesions presented in liver or spleen, loop‐mediated isothermal amplification ( LAMP and RT ‐ LAMP ) as well as PCR ‐based methods were applied. Conclusions The co‐occurrence of Ga HV ‐2 and REV genetic material was shown in 24 (96%) of 25 examined isolates. A marginal REV contamination was detected in three out 10 (30%) commercial vaccine stocks, mainly in bivalent HVT + Rispens vaccines produced between 2009 and 2012. Significance and Impact of the Study Our results indicated the common occurrence of Ga HV ‐2 and REV in Polish chicken flocks, which is probably linked to contaminated HVT + Rispens vaccine stocks. Reasons for the detection of a marginal REV contamination need to be further elucidated.