Premium
Ellagic acid derivatives from Terminalia chebula Retz. increase the susceptibility of Pseudomonas aeruginosa to stress by inhibiting polyphosphate kinase
Author(s) -
Sarabhai S.,
Harjai K.,
Sharma P.,
Capalash N.
Publication year - 2015
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12733
Subject(s) - pseudomonas aeruginosa , ellagic acid , microbiology and biotechnology , terminalia chebula , chemistry , biology , biochemistry , bacteria , antioxidant , traditional medicine , medicine , polyphenol , genetics
Aim Polyphosphate kinase 1 ( PPK 1) plays an important role in virulence, antibiotic resistance and survival under stress conditions and, therefore, is an attractive therapeutic target to control infections caused by multiple drug resistant P seudomonas aeruginosa . This study explores the PPK 1 inhibiting activity of ellagic acid derivatives ( EAD s) from T erminalia chebula Retz. that could increase the susceptibility of Ps. aeruginosa to in vitro stress conditions. Methods and Results EAD s reduced ppk1 gene expression by 93% ( P < 0·05) and completely inhibited its activity ( P < 0·01) at 0·5 mg ml −1 . EAD s‐treated Ps. aeruginosa showed marked reduction in polyphosphate granules in cytosol. Expression of rpoS , the downstream master stress response regulator, was reduced by 94% ( P < 0·05) and the sensitivity of Ps. aeruginosa increased many fold to desiccation, oxidative (H 2 O 2 ) and antibiotic (piperacillin) stresses. PPK ‐regulated swimming, swarming and twitching motilities and biofilm formation were also reduced significantly ( P ≤ 0·05) in MPAO 1 and the clinical strains of Ps. aeruginosa . Conclusion EAD s from T. chebula inhibited PPK 1 expression and its activity and increased the sensitivity of Ps. aeruginosa to desiccation and oxidative stress while reducing tolerance to piperacillin. Significance and Impact of the Study The study underlines the potential of EAD s as therapeutic agent against Ps. aeruginosa .