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Enhanced biosynthesis of O ‐desmethylangolensin from daidzein by a novel oxygen‐tolerant cock intestinal bacterium in the presence of atmospheric oxygen
Author(s) -
Li M.,
Li H.,
Zhang C.,
Wang X.L.,
Chen B.H.,
Hao Q.H.,
Wang S.Y.
Publication year - 2015
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12732
Subject(s) - strain (injury) , anaerobic exercise , bacteria , oxygen , biology , obligate anaerobe , microbiology and biotechnology , chemistry , anatomy , physiology , genetics , organic chemistry
Aims To improve the oxygen‐tolerant capability of a newly isolated anaerobic bacterium and to biosynthesize O ‐desmethylangolensin ( O ‐Dma) from daidzein aerobically. Methods and Results After a long‐term domestication process, an oxygen‐tolerant bacterium, which we named Aeroto‐ AUH ‐ JLC 108, was derived from the newly isolated obligate anaerobic bacterium Clostridium sp. AUH ‐ JLC 108. Strain Aeroto‐ AUH ‐ JLC 108 differed from the natively anaerobic wild‐type strain AUH ‐ JLC 108 by various characteristics, including a change in bacterial shape, biochemical characteristics and 16S rRNA gene sequences. Both the growth speed and the maximal optical density ( OD ) value of strain Aeroto‐ AUH ‐ JLC 108 grown aerobically were significantly increased compared to that of the wild‐type strain grown anaerobically. The maximal concentration of the substrate daidzein that the oxygen‐tolerant strain Aeroto‐AUH‐JLC108 grown aerobically was able to convert efficiently was 2·0 mmol l −1 and 0·6 mmol l −1 for strain AUH ‐ JLC 108 that was grown anaerobically. Conclusions Strain Aeroto‐ AUH ‐ JLC 108 is a conditional oxygen‐tolerant bacterium. The growth speed, bacterial growth mass and bioconversion capability of strain Aeroto‐ AUH ‐ JLC 108 grown aerobically was significantly increased compared to that of the wild‐type strain AUH ‐ JLC 108 grown anaerobically. Significance and Impact of the Study Strain Aeroto‐ AUH ‐ JLC 108 is the first reported pure culture responsible for the formation of O ‐Dma from daidzein in the presence of atmospheric oxygen.