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Endophytic fungi from the genus C olletotrichum are abundant in the P haseolus vulgaris and have high genetic diversity
Author(s) -
Gonzaga L.L.,
Costa L.E.O,
Santos T.T.,
Araújo E.F.,
Queiroz M.V.
Publication year - 2015
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12696
Subject(s) - biology , colletotrichum , colletotrichum lindemuthianum , phaseolus , botany , genetic diversity , internal transcribed spacer , plant use of endophytic fungi in defense , genetics , population , gene , ribosomal rna , demography , sociology
Aims To evaluate the diversity of endophytic fungi from the leaves of the common bean and the genetic diversity of endophytic fungi from the genus C olletotrichum using IRAP (inter‐retrotransposon amplified polymorphism) and REMAP (retrotransposon‐microsatellite amplified polymorphism) analyses. Methods and Results The fungi were isolated by tissue fragmentation and identified by analysing the morphological features and sequencing the internal transcribed spacer (ITS) regions and the rDNA large subunit (LSU). Twenty‐seven different taxa were identified. C olletotrichum was the most commonly isolated genera from the common bean (32·69% and 24·29% of the total isolates from the Ouro Negro and Talismã varieties, respectively). The IRAP and REMAP analyses revealed a high genetic diversity in the C olletotrichum endophytic isolates and were able to discriminate these isolates from the phytopathogen C olletotrichum lindemuthianum . Conclusions Fungi from the genus C olletotrichum are abundant in the P haseolus vulgaris endophytic community, and the IRAP and REMAP markers can be used to rapidly distinguish between C . lindemuthianum and other C olletotrichum members that are frequently found as endophytes. Significance and Impact of the Study This is the first report of the diversity of endophytic fungi present in the common bean and the use of IRAP and REMAP markers to assess the genetic diversity of endophytic fungi from the genus C olletotrichum .