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Cell‐bound exopolysaccharides of L actobacillus brevis KB 290 enhance cytotoxic activity of mouse splenocytes
Author(s) -
Sasaki E.,
Suzuki S.,
Fukui Y.,
Yajima N.
Publication year - 2015
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12686
Subject(s) - library science , medicine , computer science
Aims This study aimed to identify the main active component of L actobacillus brevis KB290 (KB290) that is responsible for enhanced cell‐mediated cytotoxic activity of mouse splenocytes Live KB290, a probiotic strain derived from a Japanese traditional pickle, was previously reported to modulate innate immune responses as affecting on cell‐mediated cytotoxic activity of mouse splenocytes. Methods and Results We used live KB290, heat‐killed KB290, a derivative strain ( Lact. brevis KB392) with different amounts of cell‐bound exopolysaccharide (EPS‐b), and a crude extract of EPS‐b from KB290 cell surface. Female BALB/c mice were fed a diet containing 10 10 CFU live KB290, 10 10 CFU live KB392, 15 mg heat‐killed KB290 or 600 μ g crude extract of EPS‐b for 1 day. Live KB290 ( P < 0·01), heat‐killed KB290 ( P < 0·05) and crude EPS‐b at 600 μ g ( P < 0·05) per mouse significantly enhanced cytotoxic activity; however, live KB392 had no effect. Conclusions Both live and heat‐killed KB290 and crude EPS‐b significantly enhanced cytotoxic activity of mouse splenocytes. Significance and Impact of the Study We demonstrated that EPS‐b produced by KB290 has a critical role in enhancing cell‐mediated cytotoxic activity in mouse spleen.