Premium
Polyhydroxyalkanoate ( PHA ) accumulation potential and PHA ‐accumulating microbial communities in various activated sludge processes of municipal wastewater treatment plants
Author(s) -
Sakai K.,
Miyake S.,
Iwama K.,
Inoue D.,
Soda S.,
Ike M.
Publication year - 2015
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12683
Subject(s) - polyhydroxyalkanoates , activated sludge , polyhydroxybutyrate , sewage treatment , wastewater , enhanced biological phosphorus removal , mixed liquor suspended solids , microbial population biology , pulp and paper industry , phosphorus , chemistry , resource recovery , waste management , environmental chemistry , biology , bacteria , environmental science , environmental engineering , organic chemistry , genetics , engineering
Aims To clarify the polyhydroxyalkanoate ( PHA ) accumulation potential and the PHA ‐accumulating microbial community structure in activated sludge in municipal wastewater treatment plants ( WWTP s) and to identify their influential factors. Methods and Results Nine activated sludge samples were collected from municipal WWTP s employing various biological treatment processes. In acetate‐fed 24‐h batch experiments under aerobic and nitrogen‐ and phosphorus‐limited conditions, polyhydroxybutyrate ( PHB ) content of activated sludge increased from 0–1·3 wt% to 7·9–24 wt%, with PHB yields of 0·22–0·50 C‐mol 3‐hydroxybutyrate (C‐mol acetate) −1 . Microbial community analyses found that activated sludge samples that accumulated >20 wt% of PHB after 24‐h PHA accumulation experiments had >5·0 × 10 8 copies g −1 ‐mixed liquor‐suspended solid of phaC genes. Conclusions Results indicated that (i) activated sludge in municipal WWTP s can accumulate up to approx. 20 wt% of PHA without enrichment processes, (ii) PHA accumulation potential of activated sludge varied depending on the operational conditions (treatment processes) of WWTP s, and (iii) phaC gene number can provide a simple indication of PHA accumulation potential. Significance and Impact of the Study This is the first study to compare the PHA accumulation potential and PHA ‐accumulating microbial communities in activated sludge of various treatment processes. Our findings may be useful for enhancing the resource recovery potential of wastewater treatment systems.