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Synthesis, characterization and antifungal activity of chemically and fungal‐produced silver nanoparticles against T richophyton rubrum
Author(s) -
Pereira L.,
Dias N.,
Carvalho J.,
Fernandes S.,
Santos C.,
Lima N.
Publication year - 2014
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12652
Subject(s) - trichophyton rubrum , penicillium chrysogenum , terbinafine , chemistry , silver nanoparticle , microbiology and biotechnology , minimum inhibitory concentration , nystatin , antifungal , nuclear chemistry , in vitro , itraconazole , biochemistry , biology , nanoparticle , nanotechnology , materials science
Aims To characterize and explore the potential in extracellular biosynthesis of silver nanoparticles (Ag NP s) by P enicillium chrysogenum and A spergillus oryzae and to investigate the antifungal effect of chemically vs biologically synthesized AgNPs comparing with conventional antifungal drugs against T richophyton rubrum . Methods and Results Chemically synthesized Ag NP s (Chem‐Ag NP s) coated with polyvinylpyrrolidone ( PVP ) were synthesized by chemical reduction method with glucose in PVP aqueous solution. Biologically synthesized Ag NP s (Bio‐Ag NP s) were produced from the extracellular cell‐free filtrate of P. chrysogenum MUM 03.22 and A. oryzae MUM 97.19. Among the commercial antifungal drugs, terbinafine exhibited the lower minimal inhibitory concentration ( MIC ) range values of 0·063–0·25 μ g ml −1 for the clinical strains. Chem‐Ag NP s exhibited antifungal activity against all T. rubrum strains. Bio‐Ag NP s produced by the fungal cell‐free filtrate of P. chrysogenum showed an antifungal activity higher than fluconazole but less than terbinafine, itraconazole and Chem‐Ag NP s. Conclusion The synthesis parameters in future works should be carefully studied to take full advantage of all the potential of filamentous fungi in the synthesis of Ag NP s. Significance and Impact of the Study Bio‐Ag NP s could be used as antifungal agents, namely against dermatophytes.