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Phytochemical profile of a microalgae E uglena tuba and its hepatoprotective effect against iron‐induced liver damage in S wiss albino mice
Author(s) -
Panja S.,
Chaudhuri D.,
Ghate N.B.,
Mandal N.
Publication year - 2014
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12643
Subject(s) - lipid peroxidation , phytochemical , antioxidant , chelation , chemistry , biochemistry , liver injury , pharmacology , traditional medicine , food science , biology , medicine , organic chemistry
Aims This study was aimed to evaluate different phytochemical constituents and the ameliorating effect of 70% methanol extract of Euglena tuba ( ETME ) on iron overload‐induced liver injury, along with its in vitro iron‐chelating and DNA protection effects. Methods and Results Phytochemicals of ETME were identified by GC ‐ MS analysis. Iron chelation and protection of Fenton reaction‐induced DNA damage was conducted in vitro . Post oral administration of ETME to iron‐overloaded mice, the levels of serum parameters, antioxidant enzymes, liver iron, lipid peroxidation, protein carbonyl and hydroxyproline contents were measured. ETME showed inhibition of lipid peroxidation, protein oxidation and liver fibrosis. The serum markers and liver iron were lessened, whereas enhanced levels of liver antioxidant enzymes were detected in ETME ‐treated group. Furthermore, the histopathological observations also substantiated the protective effects of the extract. Conclusions Several bioactive compounds identified by GC ‐ MS may be the basis of hepatoprotective as well as antioxidant and iron‐chelating effect of ETME . Significance and Impact of the Study Currently available iron‐chelating agents show several side effects and limitations which may be overcome by ETME , which suggest its benefit against pathology of iron overload‐linked diseases. Hence, ETME can be used as a promising hepatoprotective agent.