Premium
Environmental bacteria produce abundant and diverse antibiofilm compounds
Author(s) -
Farmer J.T.,
Shimkevitch A.V.,
Reilly P.S.,
Mlynek K.D.,
Jensen K.S.,
Callahan M.T.,
BushawNewton K.L.,
Kaplan J.B.
Publication year - 2014
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12639
Subject(s) - biofilm , bacteria , pseudomonas fluorescens , microbiology and biotechnology , staphylococcus epidermidis , staphylococcus aureus , antimicrobial , agar , biology , chemistry , pseudomonadaceae , microorganism , pseudomonas , genetics
Aims The aim of this study was to isolate novel antibiofilm compounds produced by environmental bacteria. Methods and Results Cell‐free extracts were prepared from lawns of bacteria cultured on agar. A total of 126 bacteria isolated from soil, cave and river habitats were employed. Extracts were tested for their ability to inhibit S taphylococcus aureus biofilm in a 96‐well microtitre plate assay. A total of 55/126 extracts (44%) significantly inhibited Staph. aureus biofilm. Seven extracts were selected for further analysis. The antibiofilm activities in all seven extracts exhibited unique patterns of molecular mass, chemical polarity, heat stability and spectrum of activity against Staph. aureus , S taphylococcus epidermidis and Pseudomonas fluorescens , suggesting that these seven antibiofilm activities were mediated by unique chemical compounds with different mechanisms of action. Conclusions Environmental bacteria produce abundant and diverse antibiofilm compounds. Significance and Impact of the Study Screening cell‐free extracts is a useful method for identifying secreted compounds that regulate biofilm formation. Such compounds may represent a novel source of antibiofilm agents for technological development.