Premium
In vitro fungicidal activity of biocides against pharmaceutical environmental fungal isolates
Author(s) -
Sandle T.,
Vijayakumar R.,
Saleh Al Aboody M.,
Saravanakumar S.
Publication year - 2014
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12628
Subject(s) - broth microdilution , biocide , disinfectant , cleanroom , microbiology and biotechnology , cetrimide , minimum inhibitory concentration , benzalkonium chloride , fungicide , biology , food science , chemistry , chlorhexidine , chromatography , medicine , antimicrobial , botany , materials science , organic chemistry , dentistry , nanotechnology
Aims To determine the minimum inhibitory concentrations ( MIC ) of a range of cleanroom fungi against three disinfectants common to the pharmaceutical and healthcare sectors: biguanide (chlorhexidine) and two quaternary ammonium compounds (benzalkonium chloride and cetrimide). Methods and Results The in vitro fungicidal activities of the three biocides were studied against 112 cleanroom fungal isolates using broth microdilution technique ( CLSI M38‐A2 standard). Conclusions Minimum inhibitory concentration ( MIC ) for all three biocides against hyaline fungi showed results of not more than 16 μ g ml −1 . Alternaria showed <32 μ g ml −1 and other dematiaceous fungi reported that 8–16 μ g ml −1 for biguanides and QAC s. This study clearly demonstrates that the most frequently isolated micro‐organisms from an environmental monitoring programme may be periodically subjected to broth microdilution testing with cleanroom disinfectant agents used in the disinfection programme confirm their sensitivity profile. Significance and Impact of the Study No large collection of data exists on the activity of biocides on pharmaceutical cleanroom fungal isolates. This is the first study report with large collection of cleanroom fungal isolates tested against common biocides using the broth microdilution antifungal susceptibility testing to determine the MIC value. The data presented support a quality control procedure for cleanroom disinfection.