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Do the intestinal microbiotas differ between paddlefish ( P olyodon spathala ) and bighead carp ( A ristichthys nobilis ) reared in the same pond?
Author(s) -
Li X.M.,
Zhu Y.J.,
Yan Q.Y.,
Ringø E.,
Yang D.G.
Publication year - 2014
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12626
Subject(s) - bighead carp , biology , fusobacteria , firmicutes , bacteroidetes , proteobacteria , zoology , planctomycetes , incertae sedis , carp , ecology , fishery , 16s ribosomal rna , fish <actinopterygii> , bacteria , genetics , genus
Aims A study was conducted to compare the intestinal microbial compositions of two fish species with similar feeding strategy; paddlefish ( P olyodon spathala ) and bighead carp ( A ristichthys nobilis ) reared in the same pond. Methods and Results Age‐0 paddlefish and bighead carp with mean average body lengths of 43·39 ± 2·78 and 19·33 ± 3·68 cm, respectively, were reared with natural prey items in the same pond (20 m 2 ). After 30 days of rearing, the intestinal microbiota of the two fish species was assessed by pyrosequencing of 16S rRNA genes. Interestingly, deviations were observed in the microbial communities of the two fish species according to the alpha‐ and beta‐diversity measurements and detrended correspondence analysis (DCA). Shannon diversity ( P = 0·015) and Pielou.evenness ( P = 0·035) revealed significant lower diversity of the intestinal microbiota of paddlefish. Moreover, different core intestinal microbiota was noticed in the two fish species. Proteobacteria (57·3%), Firmicutes (11·9%), Fusobacteria (8·9%), Planctomycetes (7·3%), Actinobacteria (6·0%) and Verrucomicrobia (3·2%) were detected in bighead carp, while the dominant phyla in paddlefish intestines were Bacteroidetes (37·0%), Fusobacteria (35·1%), Firmicutes (14·8%) and Proteobacteria (12·6%). Conclusions Our results revealed that the intestinal microbiota differed between paddlefish and bighead carp reared in the same pond when fed similar nature food. The potential host factors, such as the genetic background, gut histology and physiology are assumed to be involved in the intestinal bacterial compositions. Significance and Impact of the Study Considering the similar feeding strategy of paddlefish and bighead carp, this study presents basic knowledge for evaluation of the importance of host factors (genetic background and gut anatomy) on intestinal microbial composition.